The same analysis can be obtained using a fluorescent staining by calcein.Ĭollaborations - image and thematic providers:Įlisabeth VAGANAY et Forence RUGGIERO, Matrix Biology and Pathology, Institut de Génomique Fonctionnelle de Lyon, ENS de lyon, Université de Lyon, CNRS UMR 5242.ĭr. The Angiogenesis Analyzer is a simple tool to quantify the ETFA experiment images by extracting characteristic information of the network.Ībove: phase contrast image of HUVEC network, analyzed by the Angiogenesis Analyzer for ImageJ. Although widely used, the interpretation of this assay still remains a problem, especially to obtain a quantitative evaluation of the vessels-like net organization. At later stage this differentiation can lead to a meshed network from different mesh sizes. In suitable culture conditions these cells form structures that can branch and mimic a pseudo capillary in vitro formation. Typically, it can detect and analyze the pseudo vascular organization of endothelial cells cultured in gel medium.Īmong the tools used to evaluate the anti-angiogenesis properties of drugs, the most used is the in vitro differentiation of primary endothelial cells culture in gel (Endothelial Tube Formation Assay (ETFA)). The " Angiogenesis Analyzer" allows analysis of cellular networks.
0 kommentar(er)
